Experiment summary for ENCSR000CVS
doi:10.17989/ENCSR000CVS
Summary
- Status
- released
- Assay
- small RNA-seq
- Biosample summary
- Homo sapiens mesenchymal stem cell of the bone marrow female adult (60 years) and male adult (57 years)
- Biosample Type
- primary cell
- Replication type
- anisogenic
- Description
- The libraries contained in this experiment come from bone marrow derived mesenchymal stem cell primary whole cells, hMSC-BM isolated from independent donors. They are stranded SE101 Illumina Hi-Seq RNA-Seq libraries from rRNA-depleted Total RNA < 200 nucleotides in size that was pre-treated with TAP prior to cloning.
- Treatments
- Tobacco Acid Pyrophosphatase for 1 hour
- Nucleic acid type
- RNA
- Depleted in
- rRNA
- Size range
- <200
- Fragmentation methods
- see document
- Strand specificity
- Strand-specific (forward)
- Platform
- Illumina HiSeq 2000
Attribution
ENCODE2 project
- Lab
- Thomas Gingeras, CSHL
- Award
- U54HG004557 (Thomas Gingeras, CSHL)
- Project
- ENCODE
- External resources
- Date submitted
- January 19, 2011
- Date released
- July 9, 2012
Anisogenic replicates
Anisogenic replicate | Technical replicate | Summary | Biosample | Library |
---|---|---|---|---|
1 | 1 | Homo sapiens female adult (60 years) mesenchymal stem of the bone marrow primary cell | ENCBS460ENC | ENCLB555AMG |
2 | 1 | Homo sapiens male adult (57 years) mesenchymal stem of the bone marrow primary cell | ENCBS459ENC | ENCLB555AMF |