Experiment summary for ENCSR000CVS

doi:10.17989/ENCSR000CVS

Summary

Status
released
Assay
small RNA-seq
Biosample summary
Homo sapiens mesenchymal stem cell of the bone marrow female adult (60 years) and male adult (57 years)
Biosample Type
primary cell
Replication type
anisogenic
Description
The libraries contained in this experiment come from bone marrow derived mesenchymal stem cell primary whole cells, hMSC-BM isolated from independent donors. They are stranded SE101 Illumina Hi-Seq RNA-Seq libraries from rRNA-depleted Total RNA < 200 nucleotides in size that was pre-treated with TAP prior to cloning.
Treatments
Tobacco Acid Pyrophosphatase for 1 hour
Nucleic acid type
RNA
Depleted in
rRNA
Size range
<200
Fragmentation methods
see document
Strand specificity
Strand-specific (forward)

Attribution

ENCODE2 project
Lab
Thomas Gingeras, CSHL
Award
U54HG004557 (Thomas Gingeras, CSHL)
Project
ENCODE
Date submitted
January 19, 2011
Date released
July 9, 2012
Tags
Search for all Experiment with internal tag ENCYCLOPEDIAv2

Anisogenic replicates

Anisogenic replicate
Technical replicate
Summary
Biosample
Library
11Homo sapiens female adult (60 years) mesenchymal stem of the bone marrow primary cellENCBS460ENCENCLB555AMG
21Homo sapiens male adult (57 years) mesenchymal stem of the bone marrow primary cellENCBS459ENCENCLB555AMF