Experiment summary for ENCSR000CRY

doi:10.17989/ENCSR000CRY

Summary

Status
released
Assay
small RNA-seq
Biosample summary
Homo sapiens HepG2 nuclear fraction
Biosample Type
cell line
Replication type
isogenic
Description
The libraries contained in this experiment come from nuclear extracts from independent growths of cell line, HepG2. They are stranded SE36 Illumina GAIIx RNA-Seq libraries from rRNA-depleted Total RNA < 200 nucleotides in size that was pre-treated with TAP prior to cloning.
Treatments
Tobacco Acid Pyrophosphatase for 1 hour
Nucleic acid type
RNA
Depleted in
rRNA
Size range
<200
Fragmentation methods
see document
Strand specificity
Strand-specific (forward)

Attribution

ENCODE2 project
Lab
Thomas Gingeras, CSHL
Award
U54HG004557 (Thomas Gingeras, CSHL)
Project
ENCODE
Date submitted
January 19, 2011
Date released
February 22, 2012
Tags
Search for all Experiment with internal tag ENCYCLOPEDIAv2

Isogenic replicates

Isogenic replicate
Technical replicate
Summary
Biosample
Library
21Homo sapiens HepG2 cell line nuclear fractionENCBS745AAAENCLB555AMD
11Homo sapiens HepG2 cell line nuclear fractionENCBS742AAAENCLB555AME