Experiment summary for ENCSR000CRV
doi:10.17989/ENCSR000CRV
Summary
- Status
- released
- Assay
- small RNA-seq
- Biosample summary
- Homo sapiens SK-N-SH treated with 6 μM all-trans-retinoic acid for 48 hours
- Biosample Type
- cell line
- Replication type
- isogenic
- Description
- The libraries contained in this experiment come from whole cell extracts from independent growths of cell line, SK-N-SH that was treated with retinoic acid. They are stranded SE36 Illumina GAIIx RNA-Seq libraries from rRNA-depleted Total RNA < 200 nucleotides in size that was pre-treated with TAP prior to cloning.
- Treatments
- Tobacco Acid Pyrophosphatase for 1 hour, 6 μM all-trans-retinoic acid (CHEBI:15367) for 48 hours
- Nucleic acid type
- RNA
- Depleted in
- rRNA
- Size range
- <200
- Fragmentation methods
- see document
- Strand specificity
- Strand-specific (forward)
- Platform
- Illumina Genome Analyzer IIx
Attribution
ENCODE2 project
- Lab
- Thomas Gingeras, CSHL
- Award
- U54HG004557 (Thomas Gingeras, CSHL)
- Project
- ENCODE
- External resources
- Date submitted
- January 19, 2011
- Date released
- February 22, 2012
- Reference Epigenome
- ENCSR192FFL
- Tags
Isogenic replicates
Isogenic replicate | Technical replicate | Summary | Biosample | Library |
|---|---|---|---|---|
| 1 | 1 | Homo sapiens SK-N-SH cell line treated with 6 μM all-trans-retinoic acid for 48 hours | ENCBS366ENC | ENCLB555AWK |
| 2 | 1 | Homo sapiens SK-N-SH cell line treated with 6 μM all-trans-retinoic acid for 48 hours | ENCBS367ENC | ENCLB555AWT |