Experiment summary for ENCSR000CRH

doi:10.17989/ENCSR000CRH

Summary

Status
released
Assay
small RNA-seq
Biosample summary
Homo sapiens endothelial cell of umbilical vein nuclear fraction newborn
Biosample Type
primary cell
Replication type
isogenic
Description
The libraries contained in this experiment come from umbilical vein endothelial primary nuclear extracts, HUVEC isolated from independent donors. They are stranded SE36 Illumina GAIIx RNA-Seq libraries from rRNA-depleted Total RNA < 200 nucleotides in size that was pre-treated with TAP prior to cloning.
Treatments
Tobacco Acid Pyrophosphatase for 1 hour
Nucleic acid type
RNA
Depleted in
rRNA
Size range
<200
Fragmentation methods
see document
Strand specificity
Strand-specific (forward)

Attribution

ENCODE2 project
Lab
Thomas Gingeras, CSHL
Award
U54HG004557 (Thomas Gingeras, CSHL)
Project
ENCODE
Date submitted
January 19, 2011
Date released
February 22, 2012
Annotation (physical modeling)
Submitter comment
As of August 2022, the donors associated with the biosamples in this dataset were changed from ENCDO341AAA to ENCDO466MPW and ENCDO560UMK. See PMID:32728101.
Tags
Search for all Experiment with internal tag ENCYCLOPEDIAv2

Isogenic replicates

Isogenic replicate
Technical replicate
Summary
Biosample
Library
31Homo sapiens newborn endothelial of umbilical vein primary cell nuclear fractionENCBS531ENCENCLB555ANA
41Homo sapiens newborn endothelial of umbilical vein primary cell nuclear fractionENCBS533ENCENCLB555AMZ