Experiment summary for ENCSR000CRA

doi:10.17989/ENCSR000CRA

Summary

Status
released
Assay
small RNA-seq
Biosample summary
Homo sapiens K562 nucleoplasmic fraction
Biosample Type
cell line
Replication type
isogenic
Description
The libraries contained in this experiment come from nucleoplasm extracts from independent growths of cell line, K562. They are stranded SE36 Illumina GAIIx RNA-Seq libraries from rRNA-depleted Total RNA < 200 nucleotides in size that was pre-treated with TAP prior to cloning.
Treatments
Tobacco Acid Pyrophosphatase for 1 hour
Nucleic acid type
RNA
Depleted in
rRNA
Size range
<200
Fragmentation methods
see document
Strand specificity
Strand-specific (forward)

Attribution

ENCODE2 project
Lab
Thomas Gingeras, CSHL
Award
U54HG004557 (Thomas Gingeras, CSHL)
Project
ENCODE
Date submitted
January 19, 2011
Date released
February 22, 2012
Annotation (physical modeling)
Tags
Search for all Experiment with internal tag ENCYCLOPEDIAv2

Isogenic replicates

Isogenic replicate
Technical replicate
Summary
Biosample
Library
31Homo sapiens K562 cell line nucleoplasmic fractionENCBS749AAAENCLB555AXQ
41Homo sapiens K562 cell line nucleoplasmic fractionENCBS752AAAENCLB555AXP