Experiment summary for ENCSR000CPW
doi:10.17989/ENCSR000CPW
Summary
- Status
- released
- Assay
- polyA minus RNA-seq
- Biosample summary
- Homo sapiens fibroblast of lung male adult (45 years)
- Biosample Type
- primary cell
- Replication type
- isogenic
- Description
- The libraries contained in this experiment come from lung fibroblast primary whole cells, NHLF isolated from independent donors. They are stranded PE76 Illumina GAIIx RNA-Seq libraries from rRNA-depleted and DSN normalized Poly-A- RNA > 200 nucleotides in size.
- Treatments
- duplex-specific nuclease (DSN) normalization
- Nucleic acid type
- RNA
- Depleted in
- polyadenylated mRNA, rRNA
- Size range
- >200
- Fragmentation methods
- see document
- Strand specificity
- Strand-specific (reverse)
- Spike-ins datasets
- ENCSR402QNO
- Platform
- Illumina Genome Analyzer IIx
Attribution
ENCODE2 project
- Lab
- Thomas Gingeras, CSHL
- Award
- U54HG004557 (Thomas Gingeras, CSHL)
- Project
- ENCODE
- External resources
- UCSC-ENCODE-hg19:wgEncodeEH000178
- GEO-obsolete:GSM765389
- GEO:GSE78598
- Date submitted
- July 13, 2011
- Date released
- October 17, 2011
- Tags
Isogenic replicates
Isogenic replicate | Technical replicate | Summary | Biosample | Library |
|---|---|---|---|---|
| 1 | 1 | Homo sapiens male adult (45 years) fibroblast of lung primary cell | ENCBS612ENC | ENCLB555AQS |
| 2 | 1 | Homo sapiens male adult (45 years) fibroblast of lung primary cell | ENCBS613ENC | ENCLB555AQR |