Experiment summary for ENCSR000CPN

doi:10.17989/ENCSR000CPN

Summary

Status
released
Assay
polyA plus RNA-seq
Biosample summary
Homo sapiens SK-N-SH treated with 6 μM all-trans-retinoic acid for 48 hours
Biosample Type
cell line
Replication type
isogenic
Description
The libraries contained in this experiment come from the whole cell fraction of independent growths of cell line SK-N-SH that has been treated with retinoic acid. They are stranded PE76 Illumina GAIIx RNA-Seq libraries from rRNA-depleted Poly-A+ RNA > 200 nucleotides in size.
Treatments
6 μM all-trans-retinoic acid (CHEBI:15367) for 48 hours
Nucleic acid type
polyadenylated mRNA
Depleted in
rRNA
Size range
>200
Fragmentation methods
see document
Strand specificity
Strand-specific (reverse)
Spike-ins datasets
ENCSR402QNO

Attribution

ENCODE2 project
Lab
Thomas Gingeras, CSHL
Award
U54HG004557 (Thomas Gingeras, CSHL)
Project
ENCODE
External resources
Date submitted
October 17, 2011
Date released
October 17, 2011
Reference Epigenome
ENCSR192FFL 
Tags
Search for all Experiment with internal tag ENCYCLOPEDIAv1Search for all Experiment with internal tag ENCYCLOPEDIAv2

Isogenic replicates

Isogenic replicate
Technical replicate
Summary
Biosample
Library
11Homo sapiens SK-N-SH cell line treated with 6 μM all-trans-retinoic acid for 48 hoursENCBS366ENCENCLB555AOG
21Homo sapiens SK-N-SH cell line treated with 6 μM all-trans-retinoic acid for 48 hoursENCBS367ENCENCLB555ARW