Experiment summary for ENCSR000AJG
doi:10.17989/ENCSR000AJG
Summary
- Status
- released
- Assay
- single-cell RNA sequencing assay (scRNA-seq)
- Biosample summary
- Homo sapiens GM12878
- Biosample Type
- cell line
- Replication type
- isogenic
- Description
- RNA-seq of a GM12878 10-11 cell pool
- Nucleic acid type
- RNA
- Library starting quantity
- 10cells [1-1]11cells [2-1]
- Size range
- >200
- Lysis method
- 72 degrees for 3 minutes in the presence of Triton
- Fragmentation methods
- chemical (Nextera tagmentation)
- Size selection method
- SPRI beads
- Strand specificity
- unstranded
- Spike-ins datasets
- ENCSR013YHQ
- Platform
- Illumina HiSeq 2000
Attribution
ENCODE3 project
- Lab
- Barbara Wold, Caltech
- Award
- U54HG006998 (Richard Myers, HAIB)
- Project
- ENCODE
- External resources
- References
- Aliases
- barbara-wold:single-cell-13300-exp
- Date submitted
- May 13, 2014
- Date released
- June 30, 2014
- Submitter comment
- Estimates of technical measurement error from low input mass RNA-seq libraries.
Isogenic replicates
Isogenic replicate | Technical replicate | Summary | Biosample | Library |
|---|---|---|---|---|
| 1 | 1 | Homo sapiens GM12878 cell line | ENCBS934CYZ | ENCLB550GYC |
| 2 | 1 | Homo sapiens GM12878 cell line | ENCBS236ZAD | ENCLB576ZGP |