Experiment summary for ENCSR000AIZ

doi:10.17989/ENCSR000AIZ

Summary

Status
released
Assay
single-cell RNA sequencing assay (scRNA-seq)
Biosample summary
Homo sapiens GM12878
Biosample Type
cell line
Replication type
isogenic
Description
RNA-seq of a GM12878 30 cell pool
Nucleic acid type
RNA
Library starting quantity
30cells
Size range
>200
Lysis method
72 degrees for 3 minutes in the presence of Triton
Fragmentation methods
chemical (Nextera tagmentation)
Size selection method
SPRI beads
Strand specificity
unstranded
Spike-ins datasets
ENCSR193ZXE

Attribution

ENCODE3 project
Lab
Barbara Wold, Caltech
Award
U54HG006998 (Richard Myers, HAIB)
Project
ENCODE
External resources
Date submitted
July 30, 2014
Date released
September 29, 2014
Submitter comment
Estimates of technical measurement error from low input mass RNA-seq libraries.

Isogenic replicates

Isogenic replicate
Technical replicate
Summary
Biosample
Library
11Homo sapiens GM12878 cell lineENCBS791FEHENCLB482LEX
21Homo sapiens GM12878 cell lineENCBS839NNFENCLB898RJN