ENCBS998NEJ / whole organisms
Summary
- Status
- released
- Term name
- whole organism
- Term ID
- UBERON:0000468
- Summary
- Drosophila melanogaster strain Hr78-GFP wandering third instar larva whole organism stably expressing C-terminal eGFP-tagged Hr78
- Life stage
- wandering third instar larva
- Age
- Unknown
Attribution
modENCODE Project
- Lab
- Kevin White, UChicago
- Award PI
- Kevin White, UChicago
- Submitted by
- Kevin White
- Source
- Kevin White
- Project
- modENCODE
- Aliases
- kevin-white:B1-modencode_5000
Genetic modifications
Accession | Category | Purpose | Method | Nucleic acid delivery method | Site |
|---|---|---|---|---|---|
| ENCGM419CXT | insertion | tagging | stable transfection |
|
Strain information
- Status
- released
- Accession
- ENCDO876WVT
- Aliases
- modencode:Hr78-GFP, bloomington:38653
- Donor external identifiers
- Species
- Drosophila melanogaster
- Genotype
- w[1118]; PBac{y[+mDint2] w[+mC]=Hr78-GFP.FLAG}VK00037
- Sex
- unknown
- Strain background
- VK00037
- Strain name
- Hr78-GFP
- External resources
Documents
immunofluorescence
Caption excerpt: Strain name: Hr78-GFP
Genotype: PBac{y[+]-attP-3B}VK00037
Gene…
- Caption
- Strain name: Hr78-GFP Genotype: PBac{y[+]-attP-3B}VK00037 Gene Target: Hr78 Transgene: Hr78 genomic coding region, stop codon replaced with GFP Tag Type: LAP-TAG_EGFP Short Description: P[acman] BAC number CH321-64B11 has been chosen to target the gene Hormone receptor-like in 78. The LAP-TAG containing the GFP has been recombineered in 3' of the gene in frame. The engineered vector has then been inserted into the specific docking site with the help of Att sites. Recombinant individuals were screened based on mini-white presence. The line has been stabilized as homozygous for the transgene. Vector Used: P[acman] Genomic Coordinates: 3L from 21446188 to 21533441 attP Coordinates: 2L:1582820..1582820 Made_by: Nader Jameel, Alec Victorsen, Rebecca Spokony
- Submitted by
- Kathrina Onate
- Lab
- Kevin White, UChicago
- Grant
- U01HG004264