ENCBS996JXR / cell line

Summary

Status
released
Term name
SK-N-SH
Summary
Homo sapiens SK-N-SH cell line genetically modified (insertion) using CRISPR targeting H. sapiens TOX2 treated with 6 μM all-trans-retinoic acid for 48 hours
Description
SK-N-SH cell line (treated with 6 μM all-trans-retinoic acid for 48 hours) stably expressing a C-terminal LAP-tag containing eGFP, fused to TOX2.

Attribution

ENCODE4 project
Lab
Michael Snyder, Stanford
Award PI
Michael Snyder, Stanford
Submitted by
Nathaniel Watson
Project
ENCODE
Aliases
michael-snyder:SK-N-SH_RA_eGFP-TOX2_CRISPR_ChIP_B1

Treatment details

Treatment
6 μM all-trans-retinoic acid (CHEBI:15367) for 48 hours
Type
chemical
Purpose
activation

Genetic modifications

Accession
Category
Purpose
Method
Nucleic acid delivery method
Site
ENCGM485KZTinsertiontaggingCRISPR

Donor information

Status
released
Accession
ENCDO000ABD
Aliases
encode:donor of SK-N-SH, bradley-bernstein:Donor of SK-N-SH cells, encode:donor of SH-SY5Y
Species
Homo sapiens
Life stage
child
Age
4 years
Sex
female
Health status
Metastatic neuroblastoma from bone marrow
External resources
References

Documents

immunoblot
Caption excerpt: Immunoprecipitation was performed on nuclear extracts from biosample…
Attachment from submitter
Caption
Immunoprecipitation was performed on nuclear extracts from biosample ENCBS996JXR (SK-N-SH eGFP-TOX2 replicate 1) cells using anti-eGFP antibody. The image shows western blot analysis of input (lane 1), immunoprecipitate (lane 2), and mock immunoprecipitate using IgG (lane 3). Molecular weight standard (Bio-Rad, cat. # 161-0374) contains 10 pre-stained recombinant proteins (250, 150, 100, 75, 50, 37, 25, 20, 15, and 10 kD). The target molecular weight is 80.0 kD as indicated with an arrow.
Submitted by
Nathaniel Watson
Lab
Michael Snyder, Stanford