ENCBS984RGW / cell line
Summary
- Status
- released
- Term name
- MCF-7
- Term ID
- EFO:0001203
- Summary
- Homo sapiens MCF-7 cell line stably expressing C-terminal eGFP-tagged KLF4
- Description
- MCF7 cell line stably expressing a C-terminal LAP-tag containing eGFP, fused to KLF4
- Culture start date
- 2016-08-26
- Culture harvest date
- 2016-11-15
- Passage number
- 5
Attribution
ENCODE3 project
- Lab
- Kevin White, UChicago
- Award PI
- Michael Snyder, Stanford
- Submitted by
- Alec Victorsen
- Source
- Kevin White
- Project
- ENCODE
- External resources
- Aliases
- kevin-white:KLF4_GFP_MCF7_Rep1
Genetic modifications
Accession | Category | Purpose | Method | Nucleic acid delivery method | Site |
|---|---|---|---|---|---|
| ENCGM952SDA | insertion | tagging | stable transfection |
|
Donor information
- Status
- released
- Accession
- ENCDO000AAE
- Aliases
- encode:donor of MCF-7, bradley-bernstein:Donor of MCF-7 cells
- Species
- Homo sapiens
- Life stage
- adult
- Age
- 69 years
- Sex
- female
- Health status
- breast cancer (adenocarcinoma)
- Ethnicity
- European
- External resources
- References
Documents
immunoblot
Caption: immunoblot of GFP tagged KLF4, expressed in MCF7 cells
- Submitted by
- Alec Victorsen
- Lab
- Kevin White, UChicago
- Grant
- U54HG006996
immunofluorescence
Caption: immunofluorescence of GFP tagged KLF4 in MCF7 cells
- Submitted by
- Alec Victorsen
- Lab
- Kevin White, UChicago
- Grant
- U54HG006996
general protocol
Description: Protocol for generating GFP tagged BAC transgenic lines in MCF7.
- Submitted by
- Alec Victorsen
- Lab
- Kevin White, UChicago
- Grant
- U54HG006996