ENCBS967LSP / whole organisms

Summary

Status
released
Term name
whole organism
Summary
Caenorhabditis elegans strain OP749 (wgIs749 [tlf-1::TY1::EGFP::3xFLAG + unc-119(+)];unc-119 (tm4063)) mixed sex young adult (65.5 hours) at L1 larva starved after bleaching stage whole organism genetically modified (insertion) using bombardment targeting eGFP-tlf-1
Description
Transgenic C. elegans line, stably expressing a C-terminal GFP-FLAG tag fused to TLF-1.
Life stage
young adult
Age
65.5 hours
Synchronization timepoint
L1 larva starved after bleaching

Attribution

modERN project
Lab
Valerie Reinke, Yale
Award PI
Robert Waterston, UW
Submitted by
Alec Victorsen
Project
modERN
Aliases
valerie-reinke:TLF-1_GFP_OP749_yAd_Rep1

Genetic modifications

AccessionCategoryPurposeMethodSite
ENCGM853GKKinsertiontaggingbombardment
Integration site
random

Strain information

Status
released
Accession
ENCDO441DRT
Aliases
robert-waterston:OP749
Donor external identifiers
Species
Caenorhabditis elegans
Genotype
wgIs749 [tlf-1::TY1::EGFP::3xFLAG + unc-119(+)];unc-119 (tm4063)
Sex
mixed
Strain name
OP749

Documents

immunofluorescence
Caption excerpt: DIC and GFP image of transgenic worm taken right before collection. Worms…
Attachment from submitter
Caption
DIC and GFP image of transgenic worm taken right before collection. Worms imaged using a Zeiss Axioplan 2 imaging epifluorescence microscope. Scale bars are 10 micron.
Submitted by
Alec Victorsen
Lab
Valerie Reinke, Yale