ENCBS952IEM / whole organisms
Summary
- Status
- released
- Term name
- whole organism
- Term ID
- UBERON:0000468
- Summary
- Caenorhabditis elegans strain OP749 (wgIs749 [tlf-1::TY1::EGFP::3xFLAG + unc-119(+)];unc-119 (tm4063)) mixed sex young adult (65.5 hours) at L1 larva starved after bleaching stage whole organism genetically modified (insertion) using bombardment targeting C. elegans tlf-1
- Description
- Transgenic C. elegans line, stably expressing a C-terminal GFP-FLAG tag fused to TLF-1.
- Life stage
- young adult
- Age
- 65.5 hours
- Synchronization timepoint
- L1 larva starved after bleaching
Attribution
modERN project
- Lab
- Valerie Reinke, Yale
- Award PI
- Robert Waterston, UW
- Submitted by
- Alec Victorsen
- Source
- Valerie Reinke
- Project
- modERN
- External resources
- Aliases
- valerie-reinke:TLF-1_GFP_OP749_yAd_INPUT1
Genetic modifications
Accession | Category | Purpose | Method | Nucleic acid delivery method | Site |
|---|---|---|---|---|---|
| ENCGM853GKK | insertion | tagging | bombardment |
|
Strain information
- Status
- released
- Accession
- ENCDO441DRT
- Aliases
- robert-waterston:OP749
- Donor external identifiers
- Species
- Caenorhabditis elegans
- Genotype
- wgIs749 [tlf-1::TY1::EGFP::3xFLAG + unc-119(+)];unc-119 (tm4063)
- Sex
- mixed
- Strain name
- OP749
- External resources
Documents
immunofluorescence
Caption excerpt: DIC and GFP image of transgenic worm taken right before collection. Worms…
- Caption
- DIC and GFP image of transgenic worm taken right before collection. Worms imaged using a Zeiss Axioplan 2 imaging epifluorescence microscope. Scale bars are 10 micron.
- Submitted by
- Alec Victorsen
- Lab
- Valerie Reinke, Yale
- Grant
- U41HG007355