ENCBS951MKM / primary cell
Summary
- Status
- released
- Term name
- activated T-helper 1 cell
- Term ID
- NTR:0000633
- Summary
- Homo sapiens male adult (35 years) activated T-helper 1 primary cell treated with anti-CD3 and anti-CD28 coated beads for 24 hours, 30 ng/mL Interleukin-12 subunit beta for 36 hours, 10 ng/mL Interleukin-2 for 14 days, anti-CD3 and anti-CD28 coated beads for 14 days, 1 μg/mL Interleukin-4 antibody for 36 hours, 30 ng/mL Interleukin-12 subunit alpha for 36 hours
- Starting amount
- 2000000cells
- Separated from biosample
- ENCBS992HUL
Attribution
ENCODE4 project
- Lab
- Charles Lee
- Award PI
- Yijun Ruan, JAX
- Submitted by
- Khine Lin
- Source
- AllCells
- Project
- ENCODE
- Aliases
- erez-aiden:CD4-Naive-(CD45RO-CCR7+)-Day13-Activation_Th1_LP-18_4
Treatment details
- Treatment
- anti-CD3 and anti-CD28 coated beads for 24 hours
- Type
- antibody
- Purpose
- activation
- Treatment
- 30 ng/mL Interleukin-12 subunit beta (UniProtKB:P29460) for 36 hours
- Type
- protein
- Purpose
- activation
- Treatment
- 10 ng/mL Interleukin-2 (UniProtKB:P60568) for 14 days
- Type
- protein
- Purpose
- stimulation
- Treatment
- anti-CD3 and anti-CD28 coated beads for 14 days
- Type
- antibody
- Purpose
- activation
- Treatment
- 1 μg/mL Interleukin-4 antibody for 36 hours
- Type
- antibody
- Purpose
- activation
- Treatment
- 30 ng/mL Interleukin-12 subunit alpha (UniProtKB:P29459) for 36 hours
- Type
- protein
- Purpose
- activation
Donor information
- Status
- released
- Accession
- ENCDO374BBL
- Aliases
- yijun-ruan:donor_LP-18
- Donor external identifiers
- AllCells:10477
- Species
- Homo sapiens
- Life stage
- adult
- Age
- 35 years
- Sex
- male
- External resources
- Submitter comment
- AllCells:10477
Documents
cell isolation protocol
Description excerpt: Protocol describing the purification, activation, and differentiation of…
- Description
- Protocol describing the purification, activation, and differentiation of various immune cells. This document is an updated version of /documents/f1b60b4e-b5d2-4b8d-af0b-06b767fa07c3/.
- Submitted by
- Jennifer Jou
- Lab
- Charles Lee
- Grant
- UM1HG009409