ENCBS948XBF / cell line

Summary

Status
released
Term name
MCF-7
Summary
Homo sapiens MCF-7 cell line stably expressing C-terminal eGFP-tagged CEBPG
Description
MCF7 cell line stably expressing a C-terminal LAP-tag containing eGFP, fused to CEBPG
Culture start date
2015-05-06

Attribution

ENCODE3 project
Lab
Kevin White, UChicago
Award PI
Michael Snyder, Stanford
Submitted by
Alec Victorsen
Project
ENCODE
Aliases
kevin-white:CEBPG_GFP_MCF7_Rep1

Genetic modifications

Accession
Category
Purpose
Method
Nucleic acid delivery method
Site
ENCGM625JAWinsertiontaggingstable transfection
Coordinates
hg19 chr19:33642389-33900257

Donor information

Status
released
Accession
ENCDO000AAE
Aliases
encode:donor of MCF-7, bradley-bernstein:Donor of MCF-7 cells
Species
Homo sapiens
Life stage
adult
Age
69 years
Sex
female
Health status
breast cancer (adenocarcinoma)
Ethnicity
Caucasian
External resources
References

Documents

immunoblot
Caption: immunoblot of GFP tagged CEBPG, expressed in MCF7 cells
Attachment from submitter
Submitted by
Alec Victorsen
Lab
Kevin White, UChicago
immunofluorescence
Caption: immunofluorescence of GFP tagged CEBPG in MCF7 cells
Attachment from submitter
Submitted by
Alec Victorsen
Lab
Kevin White, UChicago
general protocol
Description: Protocol for generating GFP tagged BAC transgenic lines in MCF7.
Submitted by
Alec Victorsen
Lab
Kevin White, UChicago