ENCBS947VKK / cell line
Summary
- Status
- released
- Term name
- K562
- Term ID
- EFO:0002067
- Summary
- Homo sapiens K562 cell line expressing RNAi targeting H. sapiens PTBP1
- Description
- The continuous cell line K-562 was established by Lozzio and Lozzio from the pleural effusion of a 53-year-old female with chronic myelogenous leukemia in terminal blast crises. This K562 growth has been treated with an shRNA against PTBP1
- Product ID
- CCL-243
- Lot ID
- 59300853
Attribution
ENCODE3 project
- Lab
- Brenton Graveley, UConn
- Award PI
- Brenton Graveley, UConn
- Submitted by
- Brenton Graveley
- Source
- ATCC
- Project
- ENCODE
- External resources
- Aliases
- brenton-graveley:BGK0018B-5, brenton-graveley:K-PTBP1-1
- Tags
Genetic modifications
Accession | Category | Purpose | Method | Nucleic acid delivery method | Site |
|---|---|---|---|---|---|
| ENCGM637CPI | interference | repression | RNAi | transduction |
|
Donor information
- Status
- released
- Accession
- ENCDO000AAD
- Aliases
- encode:donor of K562, bradley-bernstein:Donor of K562 cells
- Species
- Homo sapiens
- Life stage
- adult
- Age
- 53 years
- Sex
- female
- Health status
- chronic myelogenous leukemia (CML)
- External resources
- References
Documents
growth protocol
Description: Growth protocol for K562 cells used in the RNA evaluation dataset for ENCODE
- Submitted by
- Brenton Graveley
- Lab
- Brenton Graveley, UConn
- Grant
- U54HG007005
transfection protocol
Description: Transfection protocol for shRNA transfection in K562 cells
- Submitted by
- Brenton Graveley
- Lab
- Brenton Graveley, UConn
- Grant
- U54HG007005