ENCBS937IYB / whole organisms

Summary

Status
released
Term name
whole organism
Summary
Caenorhabditis elegans strain OP598 (unc-119(tm4063) III; wgIs598) mixed sex young adult 63 hours post synchronization at L1 larva starved after bleaching stage whole organism genetically modified (insertion) using bombardment targeting C. elegans T26A5.8
Description
Transgenic C. elegans line, stably expressing a C-terminal GFP-FLAG tag fused to T26A5.8
Life stage
young adult
Age
Unknown
Synchronization timepoint
L1 larva starved after bleaching + 63.0 hours

Attribution

modERN project
Lab
Valerie Reinke, Yale
Award PI
Robert Waterston, UW
Submitted by
Alec Victorsen
Project
modERN
External resources
Aliases
valerie-reinke:T26A5.8_GFP_OP598_yAd_Rep4

Genetic modifications

Accession
Category
Purpose
Method
Nucleic acid delivery method
Site
ENCGM663GNFinsertiontaggingbombardment
Coordinates
ce11 chrIII:6450914-6482872
Integration site
random

Strain information

Status
released
Accession
ENCDO736PVE
Donor external identifiers
Species
Caenorhabditis elegans
Genotype
unc-119(tm4063) III; wgIs598
Sex
mixed
Strain name
OP598
External resources

Documents

immunofluorescence
Caption excerpt: DIC and GFP image of transgenic worm taken right before collection. Worms…
Attachment from submitter
Caption
DIC and GFP image of transgenic worm taken right before collection. Worms imaged using a Zeiss Axioplan 2 imaging epifluorescence microscope. Scale bars are 10 micron.
Submitted by
Alec Victorsen
Lab
Valerie Reinke, Yale
general protocol
Description: Protocol used by the Reinke lab for ChIP-seq
Submitted by
Alec Victorsen
Lab
Valerie Reinke, Yale