ENCBS925NOW / cell line

Summary

Status
released
Term name
K562
Summary
Homo sapiens K562 cell line genetically modified (insertion) using CRISPR targeting E2F5
Description
K562 cell line stably expressing a C-terminal LAP-tag containing eGFP, fused to E2F5
Culture start date
2015-11-18
Culture harvest date
2015-12-21
Passage number
5

Attribution

ENCODE3 project
Lab
Kevin White, UChicago
Award PI
Michael Snyder, Stanford
Submitted by
Alec Victorsen
Project
ENCODE
Aliases
kevin-white:E2F5_CRISPR_GFP_K562_Rep1

Genetic modifications

Accession
Category
Purpose
Method
Nucleic acid delivery method
Site
ENCGM704LNMinsertiontaggingCRISPR

Donor information

Status
released
Accession
ENCDO000AAD
Aliases
encode:donor of K562, bradley-bernstein:Donor of K562 cells
Species
Homo sapiens
Life stage
adult
Age
53 years
Sex
female
Health status
chronic myelogenous leukemia (CML)
External resources
References

Documents

immunofluorescence
Caption: immunofluorescence of GFP tagged E2F5 in K562 cells
Attachment from submitter
Submitted by
Alec Victorsen
Lab
Kevin White, UChicago
immunoblot
Caption: immunoblot of GFP tagged E2F5, expressed in K562 cells
Attachment from submitter
Submitted by
Alec Victorsen
Lab
Kevin White, UChicago
general protocol
Description: Protocol for generating GFP tagged transgenic lines in K562 by CRISPR
Submitted by
Alec Victorsen
Lab
Kevin White, UChicago