ENCBS904WOZ / cell line

Summary

Status
released
Term name
MDA-MB-231
Summary
Homo sapiens MDA-MB-231 cell line genetically modified (insertion) using transduction, genetically modified using CRISPRi (pgRNA) 14 days after the sample was treated with 2 μg/mL Puromycin for 2 days, 0.2 μg/mL Puromycin for 2 days
Culture start date
2019-05-25
Culture harvest date
2019-07-10

Attribution

ENCODE4 project
Lab
Bing Ren, UCSD
Award PI
Yin Shen, UCSF
Submitted by
Bin Li
Source
ATCC
Project
ENCODE
Aliases
bing-ren:BC19B_MB231_D14_B1_BC127

Treatment details

Treatment
2 μg/mL Puromycin (CHEBI:17939) for 2 days
Type
chemical
Purpose
selection
Treatment
0.2 μg/mL Puromycin (CHEBI:17939) for 2 days
Type
chemical
Purpose
selection

Genetic modifications

Accession
Category
Purpose
Method
Nucleic acid delivery method
Site
ENCGM554RTSinsertionexpressiontransduction
ENCGM353IMQCRISPRicharacterizationCRISPR

Donor information

Status
released
Accession
ENCDO346WJP
Aliases
encode:donor_MDA-MB-231
Species
Homo sapiens
Life stage
adult
Age
51 years
Sex
female
Ethnicity
European