ENCBS903OGK / cell line
Summary
- Status
- released
- Term name
- K562
- Term ID
- EFO:0002067
- Summary
- Homo sapiens K562 cell line genetically modified (deletion) using CRISPR targeting H. sapiens HNRNPF
- Description
- RNA-seq on K562 cells treated with a CRISPR gRNA against HNRNPF. (HNRNPF_BGKcLV02-9)
- Product ID
- CCL-243
- Lot ID
- 59300853
Attribution
ENCODE3 project
- Lab
- Brenton Graveley, UConn
- Award PI
- Brenton Graveley, UConn
- Submitted by
- Xintao Wei
- Source
- ATCC
- Project
- ENCODE
- External resources
- Aliases
- brenton-graveley:HNRNPF_BGKcLV02-9
- Tags
Genetic modifications
Accession | Category | Purpose | Method | Nucleic acid delivery method | Site |
|---|---|---|---|---|---|
| ENCGM334LMB | deletion | repression | CRISPR |
|
Donor information
- Status
- released
- Accession
- ENCDO000AAD
- Aliases
- encode:donor of K562, bradley-bernstein:Donor of K562 cells
- Species
- Homo sapiens
- Life stage
- adult
- Age
- 53 years
- Sex
- female
- Health status
- chronic myelogenous leukemia (CML)
- External resources
- References
Documents
growth protocol
Description: Growth protocol for K562 cells used in the RNA evaluation dataset for ENCODE
- Submitted by
- Brenton Graveley
- Lab
- Brenton Graveley, UConn
- Grant
- U54HG007005
treatment protocol
Description: CRISPR kd protocol for HNRNPF in K562
- Submitted by
- Jean Davidson
- Lab
- Brenton Graveley, UConn
- Grant
- U54HG007005
general protocol
Description excerpt: Graveley Lab CRISPR Genome Editing followed by RNA-seq Biosample Preparation…
- Description
- Graveley Lab CRISPR Genome Editing followed by RNA-seq Biosample Preparation and Characterization Document
- Submitted by
- Zachary Myers
- Lab
- Brenton Graveley, UConn
- Grant
- U54HG007005