ENCBS879FCF / whole organisms

Summary

Status
released
Term name
whole organism
Summary
Caenorhabditis elegans strain OP700 (wgIs700(drap-1::TY1 EGFP 3xFLAG(p_wTRG5_insertion_c_0086_1E01);unc-119(+));unc-119 (tm4063)) mixed sex mixed stage (embryo) (3 hours) using egg bleaching whole organism genetically modified (insertion) using bombardment targeting C. elegans drap-1
Description
Transgenic C. elegans line, stably expressing a C-terminal GFP-FLAG tag fused to DRAP-1.
Life stage
mixed stage (embryonic)
Age
3 hours
Synchronization timepoint
egg bleaching

Attribution

modERN project
Lab
Valerie Reinke, Yale
Award PI
Robert Waterston, UW
Submitted by
Alec Victorsen
Project
modERN
External resources
Aliases
valerie-reinke:DRAP-1_GFP_OP700_EM_INPUT1

Genetic modifications

Accession
Category
Purpose
Method
Nucleic acid delivery method
Site
ENCGM126PQFinsertiontaggingbombardment
Integration site
random

Strain information

Status
released
Accession
ENCDO428SCK
Aliases
robert-waterston:OP700
Species
Caenorhabditis elegans
Genotype
wgIs700(drap-1::TY1 EGFP 3xFLAG(p_wTRG5_insertion_c_0086_1E01);unc-119(+));unc-119 (tm4063)
Sex
mixed
Strain name
OP700
External resources

Documents

immunofluorescence
Caption excerpt: DIC and GFP image of transgenic worm taken right before collection. Worms…
Attachment from submitter
Caption
DIC and GFP image of transgenic worm taken right before collection. Worms imaged using a Zeiss Axioplan 2 imaging epifluorescence microscope. Scale bars are 10 micron.
Submitted by
Alec Victorsen
Lab
Valerie Reinke, Yale