ENCBS865CYA / cell line

Summary

Status
released
Term name
SW620
Summary
Homo sapiens SW620 cell line genetically modified (insertion) using transduction, genetically modified (interference) using CRISPR (sgRNA) 0 days after the sample was treated with 2 μg/mL puromycin for 2 days, 0.2 μg/mL puromycin for 2 days
Culture start date
2019-05-25
Culture harvest date
2019-07-10

Attribution

ENCODE4 project
Lab
Bing Ren, UCSD
Award PI
Yin Shen, UCSF
Submitted by
Bin Li
Source
ATCC
Project
ENCODE
Aliases
bing-ren:BC19B_SW620_D0_B1_BC121

Treatment details

Treatment
2 μg/mL puromycin (CHEBI:17939) for 2 days
Type
chemical
Treatment
0.2 μg/mL puromycin (CHEBI:17939) for 2 days
Type
chemical

Genetic modifications

Accession
Category
Purpose
Method
Nucleic acid delivery method
Site
ENCGM554RTSinsertionexpressiontransduction
ENCGM353IMQinterferencecharacterizationCRISPR

Donor information

Status
released
Accession
ENCDO499CXJ
Aliases
john-stamatoyannopoulos:ATCC_SW-480, encode:donor of SW480
Species
Homo sapiens
Life stage
adult
Age
50 years
Sex
male
Ethnicity
Caucasian
External resources