ENCBS847YWI / whole organisms

Summary

Status
released
Term name
whole organism
Summary
Caenorhabditis elegans strain IG1654 (frSi12[pNP157(akir-1p::AKIR-1::GFP)]II) mixed sex young adult (70 hours) at L1 larva starved after bleaching stage whole organism genetically modified (insertion) using CRISPR targeting eGFP-akir-1
Description
Transgenic C. elegans line, stably expressing a C-terminal GFP-FLAG tag fused to AKIR-1.
Life stage
young adult
Age
70 hours
Synchronization timepoint
L1 larva starved after bleaching

Attribution

modERN project
Lab
Valerie Reinke, Yale
Award PI
Robert Waterston, UW
Submitted by
Alec Victorsen
Project
modERN
Aliases
valerie-reinke:AKIR-1_GFP_IG1654_yAd_Rep3

Genetic modifications

AccessionCategoryPurposeMethodSite
ENCGM524HUGinsertiontaggingCRISPR
Coordinates
ce11 chrII:8420207-8420207

Strain information

Status
released
Accession
ENCDO518KHK
Aliases
robert-waterston:IG1654
Species
Caenorhabditis elegans
Genotype
frSi12[pNP157(akir-1p::AKIR-1::GFP)]II
Sex
mixed
Strain name
IG1654

Documents

immunofluorescence
Caption excerpt: DIC and GFP image of transgenic worm taken right before collection. Worms…
Attachment from submitter
Caption
DIC and GFP image of transgenic worm taken right before collection. Worms imaged using a Zeiss Axioplan 2 imaging epifluorescence microscope. Scale bars are 10 micron.
Submitted by
Alec Victorsen
Lab
Valerie Reinke, Yale