ENCBS847DWP / cell line

Summary

Status
released
Term name
A549
Summary
Homo sapiens A549 cell line genetically modified (insertion) using CRISPR targeting H. sapiens HOXB13
Culture harvest date
2018-07-20
Separated from biosample
ENCBS889WJU

Attribution

ENCODE4 project
Lab
Michael Snyder, Stanford
Award PI
Michael Snyder, Stanford
Submitted by
Nathaniel Watson
Project
ENCODE
Aliases
michael-snyder:pB-11151, michael-snyder:pB-eGFP-HOXB13 A549 rep6 (2)

Genetic modifications

Accession
Category
Purpose
Method
Nucleic acid delivery method
Site
ENCGM204LLJinsertiontaggingCRISPR

Donor information

Status
released
Accession
ENCDO000AAZ
Aliases
encode:donor of A549, bradley-bernstein:Donor of A549 cells, john-stamatoyannopoulos:ATCC_A549
Species
Homo sapiens
Life stage
adult
Age
58 years
Sex
male
Ethnicity
European
External resources
References

Documents

growth protocol
Description: Cell growth protocol for A549 cells (version 1).
Submitted by
Nathaniel Watson
Lab
Michael Snyder, Stanford
transfection protocol
Description excerpt: A549 transfection protocol - Version 1 (Replicates split right after…
Description
A549 transfection protocol - Version 1 (Replicates split right after transfection, 500 µg/ml G418)
Submitted by
Nathaniel Watson
Lab
Michael Snyder, Stanford