ENCBS847DWP / cell line
Summary
- Status
- released
- Term name
- A549
- Term ID
- EFO:0001086
- Summary
- Homo sapiens A549 cell line genetically modified (insertion) using CRISPR targeting H. sapiens HOXB13
- Culture harvest date
- 2018-07-20
- Separated from biosample
- ENCBS889WJU
Attribution
ENCODE4 project
- Lab
- Michael Snyder, Stanford
- Award PI
- Michael Snyder, Stanford
- Submitted by
- Nathaniel Watson
- Source
- Michael Snyder
- Project
- ENCODE
- External resources
- Aliases
- michael-snyder:pB-11151, michael-snyder:pB-eGFP-HOXB13 A549 rep6 (2)
Genetic modifications
Accession | Category | Purpose | Method | Nucleic acid delivery method | Site |
---|---|---|---|---|---|
ENCGM204LLJ | insertion | tagging | CRISPR |
|
Donor information
- Status
- released
- Accession
- ENCDO000AAZ
- Aliases
- encode:donor of A549, bradley-bernstein:Donor of A549 cells, john-stamatoyannopoulos:ATCC_A549
- Species
- Homo sapiens
- Life stage
- adult
- Age
- 58 years
- Sex
- male
- Ethnicity
- European
- External resources
- References
Documents
immunoblot
- Caption
- Immunoprecipitation was performed on nuclear extracts from biosample ENCBS847DWP (A549 eGFP-HOXB13 replicate 2) cells using anti-eGFP antibody.The image shows western blot analysis of input (lane 1), immunoprecipitate (lane 2), and mock immunoprecipitate using IgG (lane 3). Molecular weight standard (Bio-Rad, cat. # 161-0374) contains 10 pre-stained recombinant proteins (250, 150, 100, 75, 50, 37, 25, 20, 15, and 10 kD). The target molecular weight is 59.0 kD as indicated with an arrow. Lower size bands which may be due to potential degradation products are marked with asterisks.
- Submitted by
- Tao Wang
- Lab
- Michael Snyder, Stanford
- Grant
- UM1HG009442
- Documents
transfection protocol
- Description
- A549 transfection protocol - Version 1 (Replicates split right after transfection, 500 µg/ml G418)
- Submitted by
- Nathaniel Watson
- Lab
- Michael Snyder, Stanford
- Grant
- U54HG006996