ENCBS839DWI / cell line
Summary
- Status
- released
- Term name
- K562
- Term ID
- EFO:0002067
- Summary
- Homo sapiens K562 cell line stably expressing C-terminal eGFP-tagged GTF2A2
- Description
- K562 cell line stably expressing a C-terminal LAP-tag containing eGFP, fused to GTF2A2
- Culture start date
- 2014-05-07
- Culture harvest date
- 2014-06-30
- Passage number
- 5
Attribution
ENCODE3 project
- Lab
- Kevin White, UChicago
- Award PI
- Michael Snyder, Stanford
- Submitted by
- Alec Victorsen
- Source
- Kevin White
- Project
- ENCODE
- External resources
- Aliases
- kevin-white:GTF2A2_GFP_K562_Rep1
Genetic modifications
Accession | Category | Purpose | Method | Nucleic acid delivery method | Site |
|---|---|---|---|---|---|
| ENCGM429BRY | insertion | tagging | stable transfection |
|
Donor information
- Status
- released
- Accession
- ENCDO000AAD
- Aliases
- encode:donor of K562, bradley-bernstein:Donor of K562 cells
- Species
- Homo sapiens
- Life stage
- adult
- Age
- 53 years
- Sex
- female
- Health status
- chronic myelogenous leukemia (CML)
- External resources
- References
Documents
immunoblot
Caption: immunoblot of GFP tagged GTF2A2, expressed in K562 cells
- Submitted by
- Alec Victorsen
- Lab
- Kevin White, UChicago
- Grant
- U54HG006996
FACs analysis
Caption excerpt: eGFP-GTF2A2 tagged cells were stained with a nuclear dye, Vybrant DyeCycle…
- Caption
- eGFP-GTF2A2 tagged cells were stained with a nuclear dye, Vybrant DyeCycle Ruby Stain from Life technologies, and sorted by FACS. Shown are the cells from the GFP+ fraction quantified for their GFP and nuclear signal colocalization.
- Submitted by
- Alec Victorsen
- Lab
- Kevin White, UChicago
- Grant
- U54HG006996
FACs analysis
Caption excerpt: eGFP-GTF2A2 tagged cells were stained with a nuclear dye, Vybrant DyeCycle…
- Caption
- eGFP-GTF2A2 tagged cells were stained with a nuclear dye, Vybrant DyeCycle Ruby Stain from Life technologies, and sorted by FACS. Shown are the cells from the GFP++ fraction quantified for their GFP and nuclear signal colocalization.
- Submitted by
- Alec Victorsen
- Lab
- Kevin White, UChicago
- Grant
- U54HG006996
FACs analysis
Caption excerpt: eGFP-GTF2A2 tagged cells were stained with a nuclear dye, Vybrant DyeCycle…
- Caption
- eGFP-GTF2A2 tagged cells were stained with a nuclear dye, Vybrant DyeCycle Ruby Stain from Life technologies, and sorted by FACS. Shown are the cells from the GFP+ fraction quantified for their GFP and nuclear signal colocalization.
- Submitted by
- Alec Victorsen
- Lab
- Kevin White, UChicago
- Grant
- U54HG006996
general protocol
Description: Protocol for generating GFP tagged BAC transgenic lines in K562.
- Submitted by
- Alec Victorsen
- Lab
- Kevin White, UChicago
- Grant
- U54HG006996