ENCBS814XCI / cell line

Summary

Status
released
Term name
HCT116
Summary
Homo sapiens HCT116 cell line genetically modified (insertion) using CRISPR inserting O. sativa LOC4335696, genetically modified (insertion) using CRISPR targeting H. sapiens CTCF
Culture harvest date
2021-07-01
Separated from biosample
ENCBS335ZNA

Attribution

ENCODE4 project
Lab
Bradley Bernstein, Broad
Award PI
Bradley Bernstein, Broad
Submitted by
Siddarth Wekhande
Project
ENCODE
External resources
Aliases
bradley-bernstein:BioSam 4648

Genetic modifications

Accession
Category
Purpose
Method
Nucleic acid delivery method
Site
ENCGM628IQHinsertiontaggingCRISPR
ENCGM628AIRinsertionexpressionCRISPR
Gene

Donor information

Status
released
Accession
ENCDO000ABE
Aliases
encode:donor of HCT-116, bradley-bernstein:Donor of HCT-116 cells
Species
Homo sapiens
Life stage
adult
Age
48 years
Sex
male
Health status
colorectal carcinoma
External resources
References

Documents

Caption excerpt: This characterization is an exemption because the modification applied to the…
Attachment from submitter
Caption
This characterization is an exemption because the modification applied to the biosample is not applicable to the ChIP-seq experiment.
Submitted by
Khine Lin
Lab
ENCODE Consortium
Grant
ENCODE