ENCBS801NYS / cell line

Summary

Status
released
Term name
A549
Summary
Homo sapiens A549 cell line genetically modified (insertion) using CRISPR targeting H. sapiens HOXA7
Culture harvest date
2018-07-25
Separated from biosample
ENCBS074BKM

Attribution

ENCODE4 project
Lab
Michael Snyder, Stanford
Award PI
Michael Snyder, Stanford
Submitted by
Nathaniel Watson
Project
ENCODE
Aliases
michael-snyder:pB-11156, michael-snyder:pB-eGFP-HOXA7 A549 rep5

Genetic modifications

Accession
Category
Purpose
Method
Nucleic acid delivery method
Site
ENCGM243DSDinsertiontaggingCRISPR

Donor information

Status
released
Accession
ENCDO000AAZ
Aliases
encode:donor of A549, bradley-bernstein:Donor of A549 cells, john-stamatoyannopoulos:ATCC_A549
Species
Homo sapiens
Life stage
adult
Age
58 years
Sex
male
Ethnicity
European
External resources
References

Documents

immunoblot
Caption excerpt: Immunoprecipitation was performed on nuclear extracts from biosample…
Attachment from submitter
Caption
Immunoprecipitation was performed on nuclear extracts from biosample ENCBS801NYS (A549 eGFP-HOXA7 replicate 2) cells using anti-eGFP antibody. The image shows western blot analysis of input (lane 1), immunoprecipitate (lane 2), and mock immunoprecipitate using IgG (lane 3). Molecular weight standard (Bio-Rad, cat. # 161-0374) contains 10 pre-stained recombinant proteins (250, 150, 100, 75, 50, 37, 25, 20, 15, and 10 kD). The target molecular weight is 54.0 kD as indicated with an arrow.
Submitted by
Nathaniel Watson
Lab
Michael Snyder, Stanford
growth protocol
Description: Cell growth protocol for A549 cells (version 1).
Submitted by
Nathaniel Watson
Lab
Michael Snyder, Stanford
transfection protocol
Description excerpt: A549 transfection protocol - Version 1 (Replicates split right after…
Description
A549 transfection protocol - Version 1 (Replicates split right after transfection, 500 µg/ml G418)
Submitted by
Nathaniel Watson
Lab
Michael Snyder, Stanford