ENCBS779COV / whole organisms

Summary

Status
released
Term name
whole organism
Summary
Caenorhabditis elegans strain OP628 (wgIs628(ccch-3::TY1 EGFP 3xFLAG(p_wTRG5_insertion_c_0161_4E04);unc-119(+));unc-119 (tm4063)) mixed sex young adult (70 hours) at L1 larva starved after bleaching stage whole organism genetically modified (insertion) using bombardment targeting eGFP-ccch-3
Description
Transgenic C. elegans line, stably expressing a C-terminal GFP-FLAG tag fused to CCCH-3.
Life stage
young adult
Age
70 hours
Synchronization timepoint
L1 larva starved after bleaching

Attribution

modERN project
Lab
Valerie Reinke, Yale
Award PI
Robert Waterston, UW
Submitted by
Alec Victorsen
Project
modERN
Aliases
valerie-reinke:CCCH-3_GFP_OP628_yAd_INPUT1

Genetic modifications

AccessionCategoryPurposeMethodSite
ENCGM953PPZinsertiontaggingbombardment
Integration site
random

Strain information

Status
released
Accession
ENCDO824FHR
Aliases
robert-waterston:OP628
Species
Caenorhabditis elegans
Genotype
wgIs628(ccch-3::TY1 EGFP 3xFLAG(p_wTRG5_insertion_c_0161_4E04);unc-119(+));unc-119 (tm4063)
Sex
mixed
Strain name
OP628

Documents

immunofluorescence
Caption excerpt: DIC and GFP image of transgenic worm taken right before collection. Worms…
Attachment from submitter
Caption
DIC and GFP image of transgenic worm taken right before collection. Worms imaged using a Zeiss Axioplan 2 imaging epifluorescence microscope. Scale bars are 10 micron.
Submitted by
Alec Victorsen
Lab
Valerie Reinke, Yale