ENCBS769CFA / whole organisms
Summary
- Status
- released
- Term name
- whole organism
- Term ID
- UBERON:0000468
- Summary
- Caenorhabditis elegans strain OP754 (wgIs754 [T20F7.1::TY1::EGFP::3xFLAG + unc-119(+)];unc-119 (tm4063)) mixed sex L4 larva (48 hours) at L1 larva starved after bleaching stage whole organism genetically modified (insertion) using bombardment targeting C. elegans T20F7.1
- Description
- Transgenic C. elegans line, stably expressing a C-terminal GFP-FLAG tag fused to T20F7.1.
- Life stage
- L4 larva
- Age
- 48 hours
- Synchronization timepoint
- L1 larva starved after bleaching
Attribution
modERN project
- Lab
- Valerie Reinke, Yale
- Award PI
- Robert Waterston, UW
- Submitted by
- Alec Victorsen
- Source
- Valerie Reinke
- Project
- modERN
- External resources
- Aliases
- valerie-reinke:T20F7.1_GFP_OP754_L4_INPUT1
Genetic modifications
Accession | Category | Purpose | Method | Nucleic acid delivery method | Site |
---|---|---|---|---|---|
ENCGM394ZUL | insertion | tagging | bombardment |
|
Strain information
- Status
- released
- Accession
- ENCDO633CKI
- Aliases
- robert-waterston:OP754
- Donor external identifiers
- Species
- Caenorhabditis elegans
- Genotype
- wgIs754 [T20F7.1::TY1::EGFP::3xFLAG + unc-119(+)];unc-119 (tm4063)
- Sex
- mixed
- Strain name
- OP754
- External resources
Documents
immunofluorescence
- Caption
- DIC and GFP image of transgenic worm taken right before collection. Worms imaged using a Zeiss Axioplan 2 imaging epifluorescence microscope. Scale bars are 10 micron.
- Submitted by
- Alec Victorsen
- Lab
- Valerie Reinke, Yale
- Grant
- U41HG007355