ENCBS748ENK / whole organisms

Summary

Status
released
Term name
whole organism
Summary
Caenorhabditis elegans strain OP748 (wgIs748(ceh-6::TY1 EGFP 3xFLAG(P000007_A02_9H01);unc-119(+));unc-119 (ed3); VC2010) mixed sex late embryo (3.3 hours) using egg bleaching whole organism genetically modified (insertion) using bombardment targeting C. elegans ceh-6
Description
Transgenic C. elegans line, stably expressing a C-terminal GFP-FLAG tag fused to CEH-6.
Life stage
late embryonic
Age
3.3 hours
Synchronization timepoint
egg bleaching

Attribution

modERN project
Lab
Valerie Reinke, Yale
Award PI
Robert Waterston, UW
Submitted by
Alec Victorsen
Project
modERN
External resources
Aliases
valerie-reinke:CEH-6_GFP_OP748_LE_INPUT1

Genetic modifications

Accession
Category
Purpose
Method
Nucleic acid delivery method
Site
ENCGM418EZGinsertiontaggingbombardment
Integration site
random

Strain information

Status
released
Accession
ENCDO016WRP
Aliases
robert-waterston:OP748
Species
Caenorhabditis elegans
Genotype
wgIs748(ceh-6::TY1 EGFP 3xFLAG(P000007_A02_9H01);unc-119(+));unc-119 (ed3); VC2010
Sex
mixed
Strain name
OP748
External resources

Documents

immunofluorescence
Caption excerpt: DIC and GFP image of transgenic worm taken right before collection. Worms…
Attachment from submitter
Caption
DIC and GFP image of transgenic worm taken right before collection. Worms imaged using a Zeiss Axioplan 2 imaging epifluorescence microscope. Scale bars are 10 micron.
Submitted by
Alec Victorsen
Lab
Valerie Reinke, Yale