ENCBS731URN / cell line

Summary

Status
released
Term name
HepG2
Summary
Homo sapiens HepG2 cell line genetically modified (deletion) using CRISPR targeting H. sapiens EIF3H
Description
RNA-seq on HepG2 cells treated with a CRISPR gRNA against EIF3H. (EIF3H-BGHcLV05-16B)
Product ID
HB-8065
Lot ID
59635738
Culture start date
2016-07-05
Culture harvest date
2016-07-18

Attribution

ENCODE3 project
Lab
Brenton Graveley, UConn
Award PI
Brenton Graveley, UConn
Submitted by
Xintao Wei
Source
ATCC
Project
ENCODE
Aliases
brenton-graveley:EIF3H-BGHcLV05-16B
Tags
Visit collection page for ENCORE

Genetic modifications

Accession
Category
Purpose
Method
Nucleic acid delivery method
Site
ENCGM066YUGdeletionrepressionCRISPR

Donor information

Status
released
Accession
ENCDO000AAC
Aliases
encode:donor of HepG2, bradley-bernstein:Donor of HepG2
Species
Homo sapiens
Life stage
child
Age
15 years
Sex
male
Health status
hepatocellular carcinoma
Ethnicity
European
External resources
References

Documents

general protocol
Description: Biosample protocol for EIF3H-BGHcLV05-16B
Submitted by
Xintao Wei
Lab
Brenton Graveley, UConn
immunoblot
Caption excerpt: Western blot following CRISPR against EIF3H in HepG2 whole cell lysate using…
Attachment from submitter
Caption
Western blot following CRISPR against EIF3H in HepG2 whole cell lysate using EIF3H specific antibody. Lane 1 is a ladder, lane 2 is HepG2 non-targeting control knockdown, lane 3 and 4 are two different CRISPR against EIF3H. EIF3H protein appears as the green band, Tubulin serves as a control and appears in red.
Submitted by
Xintao Wei
Lab
Brenton Graveley, UConn