ENCBS721DUI / in vitro differentiated cells

Summary

Status
released
Term name
hematopoietic multipotent progenitor cell
Term ID
CL:0000837
Summary
Homo sapiens male adult (25 years) hematopoietic multipotent progenitor in vitro differentiated cells treated with interleukin-3 for 4 days, kit ligand for 4 days, hydrocortisone succinate for 4 days, erythropoietin for 4 days
Description
Day 4 (T4)

Attribution

ENCODE3 project
Lab
John Stamatoyannopoulos, UW
Award PI
John Stamatoyannopoulos, UW
Submitted by
Michael Buckley
Project
ENCODE
External resources
Aliases
john-stamatoyannopoulos:DS25687A

Treatment details

Treatment
interleukin-3 (UniProtKB:P08700) for 4 days
Type
protein
Purpose
stimulation
Treatment
kit ligand (UniProtKB:P21583) for 4 days
Type
protein
Purpose
stimulation
Treatment
hydrocortisone succinate (CHEBI:31677) for 4 days
Type
chemical
Purpose
stimulation
Treatment
erythropoietin (CHEBI:81579) for 4 days
Type
chemical
Purpose
stimulation

Donor information

Status
released
Accession
ENCDO937OUY
Aliases
encode:donor of RO 02366, roadmap-epigenomics:RO 02366, john-stamatoyannopoulos:RO_02366
Species
Homo sapiens
Life stage
adult
Age
25 years
Sex
male
External resources

Documents

treatment protocol
Description: Erythroid differentiation of human CD34+ cells
Submitted by
Jason Hilton
Lab
John Stamatoyannopoulos, UW
cell isolation protocol
Description: Isolation of CD34-positive cells from human leukapheresis product
Submitted by
Michael Buckley
Lab
John Stamatoyannopoulos, UW