ENCBS703ZDS / cell line
Summary
- Status
- released
- Term name
- H9
- Term ID
- EFO:0003045
- Summary
- Homo sapiens H9 cell line stably expressing C-terminal eGFP-tagged HES5
- Description
- HES5::eGFP BAC transgenic human ES cells (H9; WA-09; Wicell) expressing GFP under the HES5 promoter, Cells were cultured on mitotically inactivated mouse embryonic fibroblasts (MEFs) (Globalstem). Undifferentiated ES cells were maintained in medium containing DMEM/F12, 20% KSR, 1mM Glutamine, 1% Penicillin/Streptomycin, non essential amino acids and beta-mercaptoethanol. Undifferentiated ES cells were purified with pluripotency markers Tra-1-60 and PE-conjugated SSEA-3 (BD Pharmingen).
- Product ID
- NA
- Culture harvest date
- 2011-06-14
- Passage number
- 59
Attribution
ENCODE3 project
- Lab
- Bradley Bernstein, Broad
- Award PI
- Bradley Bernstein, Broad
- Submitted by
- Noam Shoresh
- Source
- WiCell
- Project
- ENCODE
- External resources
- Aliases
- bradley-bernstein:BioSam 1018
Genetic modifications
Accession | Category | Purpose | Method | Nucleic acid delivery method | Site |
---|---|---|---|---|---|
ENCGM252BLF | insertion | tagging | stable transfection |
|
Donor information
- Status
- released
- Accession
- ENCDO222AAA
- Aliases
- encode:donor of H9, roadmap-epigenomics:113, john-stamatoyannopoulos:H9 FUCCI, bradley-bernstein:Donor of H9 cells
- Species
- Homo sapiens
- Life stage
- embryonic
- Age
- 5 days
- Sex
- female
- Health status
- healthy
- External resources