ENCBS703ZDS / cell line

Summary

Status
released
Term name
H9
Summary
Homo sapiens H9 cell line stably expressing C-terminal eGFP-tagged eGFP-HES5
Description
HES5::eGFP BAC transgenic human ES cells (H9; WA-09; Wicell) expressing GFP under the HES5 promoter, Cells were cultured on mitotically inactivated mouse embryonic fibroblasts (MEFs) (Globalstem). Undifferentiated ES cells were maintained in medium containing DMEM/F12, 20% KSR, 1mM Glutamine, 1% Penicillin/Streptomycin, non essential amino acids and beta-mercaptoethanol. Undifferentiated ES cells were purified with pluripotency markers Tra-1-60 and PE-conjugated SSEA-3 (BD Pharmingen).
Product ID
NA
Culture harvest date
2011-06-14
Passage number
59

Attribution

ENCODE3 project
Lab
Bradley Bernstein, Broad
Award PI
Bradley Bernstein, Broad
Submitted by
Noam Shoresh
Source
WiCell
Project
ENCODE
Aliases
bradley-bernstein:BioSam 1018

Genetic modifications

AccessionCategoryPurposeMethodSite
ENCGM252BLFinsertiontaggingstable transfection

Donor information

Status
released
Accession
ENCDO222AAA
Aliases
encode:donor of H9, roadmap-epigenomics:113, john-stamatoyannopoulos:H9 FUCCI, bradley-bernstein:Donor of H9 cells
Donor external identifiers
Species
Homo sapiens
Life stage
embryonic
Age
5 days
Sex
female
Health status
healthy

Documents

general protocol
Description: H9_derived_differentiation_protocol.pdf
Submitted by
Noam Shoresh
Lab
Bradley Bernstein, Broad
other
Description excerpt: Request for Supplemental Funds to obtain differentiated stem cells for the…
Description
Request for Supplemental Funds to obtain differentiated stem cells for the ENCODE project to collectively profile
Submitted by
Julie Wong
Lab
J. Michael Cherry, Stanford
certificate of analysis
Description: WiCell: Certificate of Analysis - Amended for H9
Submitted by
Kathrina Onate
Lab
J. Michael Cherry, Stanford