ENCBS703ZDS / cell line

Summary

Status
released
Term name
H9
Summary
Homo sapiens H9 cell line stably expressing C-terminal eGFP-tagged HES5
Description
HES5::eGFP BAC transgenic human ES cells (H9; WA-09; Wicell) expressing GFP under the HES5 promoter, Cells were cultured on mitotically inactivated mouse embryonic fibroblasts (MEFs) (Globalstem). Undifferentiated ES cells were maintained in medium containing DMEM/F12, 20% KSR, 1mM Glutamine, 1% Penicillin/Streptomycin, non essential amino acids and beta-mercaptoethanol. Undifferentiated ES cells were purified with pluripotency markers Tra-1-60 and PE-conjugated SSEA-3 (BD Pharmingen).
Product ID
NA
Culture harvest date
2011-06-14
Passage number
59

Attribution

ENCODE3 project
Lab
Bradley Bernstein, Broad
Award PI
Bradley Bernstein, Broad
Submitted by
Noam Shoresh
Source
WiCell
Project
ENCODE
Aliases
bradley-bernstein:BioSam 1018

Genetic modifications

Accession
Category
Purpose
Method
Site
ENCGM252BLFinsertiontaggingstable transfection

Donor information

Status
released
Accession
ENCDO222AAA
Aliases
encode:donor of H9, roadmap-epigenomics:113, john-stamatoyannopoulos:H9 FUCCI, bradley-bernstein:Donor of H9 cells
Donor external identifiers
Species
Homo sapiens
Life stage
embryonic
Age
5 days
Sex
female
Health status
healthy

Documents

general protocol
Description: H9_derived_differentiation_protocol.pdf
Submitted by
Noam Shoresh
Lab
Bradley Bernstein, Broad
certificate of analysis
Description: WiCell: Certificate of Analysis - Amended for H9
Submitted by
Kathrina Onate
Lab
J. Michael Cherry, Stanford