ENCBS693XWV / cell line

Summary

Status
released
Term name
S2R+
Summary
Drosophila melanogaster strain Oregon-R S2R+ cell line stably expressing C-terminal FLAG-tagged Hrb87F under MtnA promoter, C-terminal HA-tagged Hrb87F under MtnA promoter treated with 0.35 mM copper(II) sulfate for 24 hours
Life stage
unknown
Age
Unknown

Attribution

modENCODE Project
Lab
Brenton Graveley, UConn
Award PI
Susan Celniker, LBNL
Submitted by
Brenton Graveley
Project
modENCODE
External resources
Aliases
brenton-graveley:B-FH3174_Blue

Treatment details

Treatment
0.35 mM copper(II) sulfate (CHEBI:23414) for 24 hours
Type
chemical

Genetic modifications

Accession
Category
Purpose
Method
Nucleic acid delivery method
Site
ENCGM914XFAinsertiontaggingstable transfection

Strain information

Status
released
Accession
ENCDO995VAO
Aliases
encode:donor of Oregon-R-modENCODE, bloomington:25211, encode:donor of Oregon-R, encode:donor of S2, encode:donor of S2-DRSC, encode:donor of S2R+, encode:donor of Sg4, encode:donor of S1, encode:donor of S3
Donor external identifiers
Species
Drosophila melanogaster
Genotype
wild type
Sex
unknown
Strain name
Oregon-R
External resources

Documents

transfection protocol
Description excerpt: Transfection_and_induction_of_FLAG-HA_tagged_contruct_into_cell_culture:SC:Grav…
Description
Transfection_and_induction_of_FLAG-HA_tagged_contruct_into_cell_culture:SC:Graveley
Submitted by
Brenton Graveley
Lab
Brenton Graveley, UConn