ENCBS686LDP / whole organisms

Summary

Status
released
Term name
whole organism
Summary
Caenorhabditis elegans strain OP646 (unc-119(tm4063) III; wgIs646) mixed sex young adult 64 hours post synchronization at L1 larva starved after bleaching stage whole organism genetically modified (insertion) using bombardment targeting C. elegans mel-28
Description
Transgenic C. elegans line, stably expressing a C-terminal GFP-FLAG tag fused to MEL-28
Life stage
young adult
Age
Unknown
Synchronization timepoint
L1 larva starved after bleaching + 64.0 hours

Attribution

modERN project
Lab
Valerie Reinke, Yale
Award PI
Robert Waterston, UW
Submitted by
Alec Victorsen
Project
modERN
External resources
Aliases
valerie-reinke:MEL-28_GFP_OP646_yAd_INPUT

Genetic modifications

Accession
Category
Purpose
Method
Nucleic acid delivery method
Site
ENCGM749TPTinsertiontaggingbombardment
Coordinates
ce11 chrIII:4763371-4798826
Integration site
random

Strain information

Status
released
Accession
ENCDO002GDY
Donor external identifiers
Species
Caenorhabditis elegans
Genotype
unc-119(tm4063) III; wgIs646
Sex
mixed
Strain name
OP646
External resources

Documents

immunofluorescence
Caption excerpt: DIC and GFP image of transgenic worm taken right before collection. Worms…
Attachment from submitter
Caption
DIC and GFP image of transgenic worm taken right before collection. Worms imaged using a Zeiss Axioplan 2 imaging epifluorescence microscope. Scale bars are 10 micron.
Submitted by
Alec Victorsen
Lab
Valerie Reinke, Yale
general protocol
Description: Protocol used by the Reinke lab for ChIP-seq
Submitted by
Alec Victorsen
Lab
Valerie Reinke, Yale