ENCBS658NLC / whole organisms

Summary

Status
revoked
Term name
whole organism
Summary
Caenorhabditis elegans strain OP494 (unc-119(tm4063) III; wgIs494) hermaphrodite late embryo 5 hours post synchronization using egg bleaching whole organism genetically modified (insertion) using bombardment targeting eGFP-ztf-16
Description
Transgenic C. elegans line, stably expressing a C-terminal GFP-FLAG tag fused to ZTF-16
Life stage
late embryonic
Age
Unknown
Synchronization timepoint
egg bleaching + 5 hours

Attribution

modERN project
Lab
Valerie Reinke, Yale
Award PI
Robert Waterston, UW
Submitted by
Alec Victorsen
Project
modERN
Aliases
valerie-reinke:ZTF-16_GFP_OP494_LE_Rep1
Submitter comment
The submitting lab requested to revoke the data associated with strain OP494 because genotyping revealed that strain contains both tagged ztf-16 and lag-1.

Genetic modifications

AccessionCategoryPurposeMethodSite
ENCGM642POYinsertiontaggingbombardment
Integration site
random

Strain information

Status
revoked
Accession
ENCDO421TEB
Donor external identifiers
Species
Caenorhabditis elegans
Genotype
unc-119(tm4063) III; wgIs494.
Sex
hermaphrodite
Strain name
OP494

Documents

immunofluorescence
Caption excerpt: DIC and GFP image of transgenic worm taken right before collection. Worms…
Attachment from submitter
Caption
DIC and GFP image of transgenic worm taken right before collection. Worms imaged using a Zeiss Axioplan 2 imaging epifluorescence microscope. Scale bars are 10 micron.
Submitted by
Alec Victorsen
Lab
Valerie Reinke, Yale