ENCBS653MZV / whole organisms
Summary
- Status
- released
- Term name
- whole organism
- Term ID
- UBERON:0000468
- Summary
- Caenorhabditis elegans strain OP740 (wgIs740 [lin-32::TY1::EGFP::3xFLAG + unc-119(+)];unc-119 (tm4063)) mixed sex mixed stage (embryo) (3 hours) using egg bleaching whole organism genetically modified (insertion) using bombardment targeting C. elegans lin-32
- Description
- Transgenic C. elegans line, stably expressing a C-terminal GFP-FLAG tag fused to LIN-32.
- Life stage
- mixed stage (embryonic)
- Age
- 3 hours
- Synchronization timepoint
- egg bleaching
Attribution
modERN project
- Lab
- Valerie Reinke, Yale
- Award PI
- Robert Waterston, UW
- Submitted by
- Alec Victorsen
- Source
- Valerie Reinke
- Project
- modERN
- External resources
- Aliases
- valerie-reinke:LIN-32_GFP_OP740_EM_Rep2
Genetic modifications
Accession | Category | Purpose | Method | Nucleic acid delivery method | Site |
|---|---|---|---|---|---|
| ENCGM282WBF | insertion | tagging | bombardment |
|
Strain information
- Status
- released
- Accession
- ENCDO944KSH
- Aliases
- robert-waterston:OP740
- Donor external identifiers
- Species
- Caenorhabditis elegans
- Genotype
- wgIs740 [lin-32::TY1::EGFP::3xFLAG + unc-119(+)];unc-119 (tm4063)
- Sex
- mixed
- Strain name
- OP740
- External resources
Documents
immunofluorescence
Caption excerpt: DIC and GFP image of transgenic worm taken right before collection. Worms…
- Caption
- DIC and GFP image of transgenic worm taken right before collection. Worms imaged using a Zeiss Axioplan 2 imaging epifluorescence microscope. Scale bars are 10 micron.
- Submitted by
- Alec Victorsen
- Lab
- Valerie Reinke, Yale
- Grant
- U41HG007355