ENCBS622BUD / whole organisms

Summary

Status
released
Term name
whole organism
Summary
Caenorhabditis elegans strain OP391 (unc-119(ed3) III; wgIs391(med-1::TY1 EGFP FLAG C; unc-119(+))) hermaphrodite midembryo whole organism genetically modified (insertion) using bombardment targeting C. elegans med-1
Description
Transgenic C. elegans line, stably expressing a C-terminal GFP-FLAG tag fused to MED-1
Life stage
midembryonic
Age
Unknown

Attribution

modERN project
Lab
Valerie Reinke, Yale
Award PI
Robert Waterston, UW
Submitted by
Alec Victorsen
Project
modERN
External resources
Aliases
valerie-reinke:MED-1_GFP_OP391_ME_INPUT

Genetic modifications

Accession
Category
Purpose
Method
Nucleic acid delivery method
Site
ENCGM933PCYinsertiontaggingbombardment
Integration site
random

Strain information

Status
released
Accession
ENCDO939KVQ
Donor external identifiers
Species
Caenorhabditis elegans
Genotype
unc-119(ed3) III; wgIs391(med-1::TY1 EGFP FLAG C; unc-119(+))
Sex
hermaphrodite
Strain name
OP391
External resources

Documents

immunofluorescence
Caption excerpt: DIC and GFP image of transgenic worm taken right before collection. Worms…
Attachment from submitter
Caption
DIC and GFP image of transgenic worm taken right before collection. Worms imaged using a Zeiss Axioplan 2 imaging epifluorescence microscope. Scale bars are 10 micron.
Submitted by
Alec Victorsen
Lab
Valerie Reinke, Yale