ENCBS620NLU / whole organisms

Summary

Status
released
Term name
whole organism
Summary
Caenorhabditis elegans strain OP344 (unc-119(tm4063); wgIs344 [F49E12.6::TY1::EGFP::3xFLAG + unc-119(+)]) mixed sex L1 larva (6 hours) at L1 larva starved after bleaching stage whole organism genetically modified (insertion) using bombardment targeting eGFP-efl-3
Description
Transgenic C. elegans line, stably expressing a C-terminal GFP-FLAG tag fused to EFL-3.
Life stage
L1 larva
Age
6 hours
Synchronization timepoint
L1 larva starved after bleaching

Attribution

modERN project
Lab
Valerie Reinke, Yale
Award PI
Robert Waterston, UW
Submitted by
Alec Victorsen
Project
modERN
Aliases
valerie-reinke:EFL-3_GFP_OP344_L1_INPUT1

Genetic modifications

AccessionCategoryPurposeMethodSite
ENCGM759HZZinsertiontaggingbombardment
Integration site
random

Strain information

Status
released
Accession
ENCDO908BUI
Donor external identifiers
Species
Caenorhabditis elegans
Genotype
unc-119(tm4063); wgIs344 [F49E12.6::TY1::EGFP::3xFLAG + unc-119(+)]
Sex
mixed
Strain name
OP344

Documents

immunofluorescence
Caption excerpt: DIC and GFP image of transgenic worm taken right before collection. Worms…
Attachment from submitter
Caption
DIC and GFP image of transgenic worm taken right before collection. Worms imaged using a Zeiss Axioplan 2 imaging epifluorescence microscope. Scale bars are 10 micron.
Submitted by
Alec Victorsen
Lab
Valerie Reinke, Yale