ENCBS617ENC / cell line

Summary

Status
released
Term name
MCF 10A
Summary
Homo sapiens MCF 10A cell line stably expressing SRC treated with 0.01% ethanol for 12 hours
Description
mammary gland, non-tumorigenic epithelial, inducible cell line, derived from the MCF-10A parental cells and contain ER-Src, a derivative of the Src kinase oncoprotein (v-Src) that is fused to the ligand-binding domain of the estrogen receptor (ER)
Product ID
MCF10A-Er-Src
Lot ID
NULL

Attribution

ENCODE2 project
Lab
Kevin Struhl, HMS
Award PI
Michael Snyder, Stanford
Submitted by
Kevin Struhl
Project
ENCODE

Treatment details

Treatment
0.01 percent ethanol (CHEBI:16236) for 12 hours
Type
chemical

Genetic modifications

Accession
Category
Purpose
Method
Nucleic acid delivery method
Site
ENCGM980FIGinsertiontaggingstable transfection
Target
SRC-human

Donor information

Status
released
Accession
ENCDO000AAP
Aliases
encode:donor of MCF10A-Er-Src, encode:donor of MCF 10A
Species
Homo sapiens
Life stage
adult
Age
36 years
Sex
female
Health status
fibrocystic disease
Ethnicity
European
External resources
References

Documents

growth protocol
Description: MCF10A-Er-Src
Submitted by
Kevin Struhl
Lab
Kevin Struhl, HMS
treatment protocol
Description: Transformation conditions of MCF10A-Er-Src
Submitted by
Kevin Struhl
Lab
Kevin Struhl, HMS