ENCBS616VAU / whole organisms

Summary

Status
released
Term name
whole organism
Summary
Caenorhabditis elegans strain ZM7247 (daf-16(mu86);daf-2(e1370) III; hpSi4II (Pmyo-3 GFP::daf-16a)) hermaphrodite L4 larva 54 hours post synchronization at L1 larva starved after bleaching stage whole organism genetically modified (insertion) using bombardment targeting C. elegans daf-16
Description
Transgenic C. elegans line, stably expressing a C-terminal GFP-FLAG tag fused to DAF-16
Life stage
L4 larva
Age
Unknown
Synchronization timepoint
L1 larva starved after bleaching + 54 hours

Attribution

modERN project
Lab
Valerie Reinke, Yale
Award PI
Robert Waterston, UW
Submitted by
Alec Victorsen
Project
modERN
Aliases
valerie-reinke:DAF-16_GFP_ZM7247_L4_Rep1

Genetic modifications

Accession
Category
Purpose
Method
Nucleic acid delivery method
Site
ENCGM225SBVinsertiontaggingbombardment
Integration site
random

Strain information

Status
released
Accession
ENCDO583CKU
Aliases
wesley-hung:ZM7247
Species
Caenorhabditis elegans
Genotype
daf-16(mu86);daf-2(e1370) III; hpSi4II (Pmyo-3 GFP::daf-16a)
Sex
hermaphrodite
Strain name
ZM7247

Documents

immunofluorescence
Caption excerpt: DIC and GFP image of transgenic worm taken right before collection. Worms…
Attachment from submitter
Caption
DIC and GFP image of transgenic worm taken right before collection. Worms imaged using a Zeiss Axioplan 2 imaging epifluorescence microscope. Scale bars are 10 micron.
Submitted by
Alec Victorsen
Lab
Valerie Reinke, Yale