ENCBS595YGG / whole organisms

Summary

Status
released
Term name
whole organism
Summary
Caenorhabditis elegans strain OP581 (unc-119(tm4063) III; wgIs581) mixed sex young adult 54.5 hours post synchronization at L1 larva starved after bleaching stage whole organism genetically modified (insertion) using bombardment targeting C. elegans zim-3
Description
Transgenic C. elegans line, stably expressing a C-terminal GFP-FLAG tag fused to ZIM-3
Life stage
young adult
Age
Unknown
Synchronization timepoint
L1 larva starved after bleaching + 54.5 hours

Attribution

modERN project
Lab
Valerie Reinke, Yale
Award PI
Robert Waterston, UW
Submitted by
Alec Victorsen
Project
modERN
External resources
Aliases
valerie-reinke:ZIM-3_GFP_OP581_yAd_INPUT

Genetic modifications

Accession
Category
Purpose
Method
Nucleic acid delivery method
Site
ENCGM970VVTinsertiontaggingbombardment
Coordinates
ce11 chrIV:10533841-10574303
Integration site
random

Strain information

Status
released
Accession
ENCDO380GBU
Donor external identifiers
Species
Caenorhabditis elegans
Genotype
unc-119(tm4063) III; wgIs581.
Sex
mixed
Strain name
OP581
External resources

Documents

immunofluorescence
Caption excerpt: DIC and GFP image of transgenic worm taken right before collection. Worms…
Attachment from submitter
Caption
DIC and GFP image of transgenic worm taken right before collection. Worms imaged using a Zeiss Axioplan 2 imaging epifluorescence microscope. Scale bars are 10 micron.
Submitted by
Alec Victorsen
Lab
Valerie Reinke, Yale