ENCBS564SNS / whole organisms

Summary

Status
released
Term name
whole organism
Summary
Caenorhabditis elegans strain XIL99 mixed sex L1 larva at L1 larva starved after bleaching stage whole organism genetically modified (insertion) using CRISPR targeting C. elegans vab-15
Description
Transgenic C. elegans line, stably expressing a C-terminal GFP-FLAG tag fused to VAB-15
Life stage
L1 larva
Age
Unknown
Synchronization timepoint
L1 larva starved after bleaching

Attribution

modERN project
Lab
Valerie Reinke, Yale
Award PI
Robert Waterston, UW
Submitted by
Alec Victorsen
Project
modERN
External resources
Aliases
valerie-reinke:VAB-15_GFP_XIL99_L1_Rep2

Genetic modifications

Accession
Category
Purpose
Method
Nucleic acid delivery method
Site
ENCGM896RUAinsertiontaggingCRISPR
Coordinates
ce11 chrX:9849110-9849110

Strain information

Status
released
Accession
ENCDO003JWX
Aliases
robert-waterston:XIL99
Species
Caenorhabditis elegans
Sex
mixed
Strain name
XIL99
External resources

Documents

immunofluorescence
Caption excerpt: DIC and GFP image of transgenic worm taken right before collection. Worms…
Attachment from submitter
Caption
DIC and GFP image of transgenic worm taken right before collection. Worms imaged using a Zeiss Axioplan 2 imaging epifluorescence microscope. Scale bars are 10 micron.
Submitted by
Alec Victorsen
Lab
Valerie Reinke, Yale