ENCBS559ENC / cell line

Summary

Status
released
Term name
GM03348
Summary
Homo sapiens GM03348 cell line genetically modified (insertion) using transduction targeting H. sapiens MYOD1
Description
skin fibroblast; GM03348 skin fibroblasts (Coriell) were transduced with an induceable lentivirus (pAK111-TRE-3xflag hMyoD-T2A-dsRed) containing get inducible MyoD. Cells were selected in 1ug per ml puro (Sigma catalog P8833) to obtain a pure cell population. Cells were cultured for 10 days (NO DOXYCYCLINE)
Product ID
GM03348

Attribution

ENCODE2 project
Lab
Gregory Crawford, Duke
Award PI
Gregory Crawford, Duke
Submitted by
Terry Furey
Source
Coriell
Project
ENCODE

Genetic modifications

Accession
Category
Purpose
Method
Nucleic acid delivery method
Site
ENCGM883SVNinsertiontaggingtransduction

Donor information

Status
released
Accession
ENCDO186AAA
Aliases
encode:donor_of_Fibrobl_GM03348
Species
Homo sapiens
Life stage
child
Age
10 years
Sex
male
Health status
apparently healthy
Ethnicity
European

Documents

growth protocol
Description: GM03348 Fibroblast culture conditions (Coriell)
Submitted by
Gregory Crawford
Lab
Gregory Crawford, Duke