ENCBS558ENC / cell line

Summary

Status
released
Term name
GM03348
Summary
Homo sapiens GM03348 cell line genetically modified (insertion) using transduction targeting H. sapiens MYOD1 treated with 3 μg/mL doxycycline for 10 days
Description
skin fibroblast; GM03348 skin fibroblasts (Coriell) were transduced with an induceable lentivirus (pAK111-TRE-3xflag hMyoD-T2A-dsRed) containing get inducible MyoD. Cells were selected in 1ug per ml puro (Sigma catalog P8833) to obtain a pure cell population. Cells were induced for MYOD expression for 10 days with doxycycline and at concentration of 3ug per ml. Standard media was used (and replaced every 2 days) during induction: DMEM, 1% Pen Strep, and 10% FBS.
Product ID
GM03348

Attribution

ENCODE2 project
Lab
Gregory Crawford, Duke
Award PI
Gregory Crawford, Duke
Submitted by
Terry Furey
Source
Coriell
Project
ENCODE

Treatment details

Treatment
3 μg/mL doxycycline (CHEBI:50845) for 10 days
Type
chemical

Genetic modifications

Accession
Category
Purpose
Method
Nucleic acid delivery method
Site
ENCGM883SVNinsertiontaggingtransduction

Donor information

Status
released
Accession
ENCDO186AAA
Aliases
encode:donor_of_Fibrobl_GM03348
Species
Homo sapiens
Life stage
child
Age
10 years
Sex
male
Health status
apparently healthy
Ethnicity
European

Documents

growth protocol
Description: GM03348 Fibroblast culture conditions (Coriell)
Submitted by
Gregory Crawford
Lab
Gregory Crawford, Duke