ENCBS556RPW / cell line

Summary

Status
released
Term name
K562
Summary
Homo sapiens K562 cell line genetically modified (deletion) using CRISPR targeting IGF2BP1
Description
RNA-seq on K562 cells treated with a CRISPR gRNA against IGF2BP1. (IGF2BP1_BGKcLV02-4)
Product ID
CCL-243
Lot ID
59300853

Attribution

ENCODE3 project
Lab
Brenton Graveley, UConn
Award PI
Brenton Graveley, UConn
Submitted by
Xintao Wei
Source
ATCC
Project
ENCODE
External resources
Aliases
brenton-graveley:IGF2BP1_BGKcLV02-4
Tags
Search for all Biosample with internal tag ENCORE

Genetic modifications

Accession
Category
Purpose
Method
Site
ENCGM813YJCdeletionrepressionCRISPR

Donor information

Status
released
Accession
ENCDO000AAD
Aliases
encode:donor of K562, bradley-bernstein:Donor of K562 cells
Donor external identifiers
Species
Homo sapiens
Life stage
adult
Age
53 years
Sex
female
Health status
chronic myelogenous leukemia (CML)
References

Documents

growth protocol
Description: Growth protocol for K562 cells used in the RNA evaluation dataset for ENCODE
Submitted by
Brenton Graveley
Lab
Brenton Graveley, UConn
general protocol
Description excerpt: Graveley Lab CRISPR Genome Editing followed by RNA-seq Biosample Preparation…
Description
Graveley Lab CRISPR Genome Editing followed by RNA-seq Biosample Preparation and Characterization Document
Submitted by
Zachary Myers
Lab
Brenton Graveley, UConn