ENCBS549YHL / whole organisms
Summary
- Status
- released
- Term name
- whole organism
- Term ID
- UBERON:0000468
- Summary
- Caenorhabditis elegans strain ZM8745 (daf-16(mu86);daf-2(e1370) III; hpSi15 (Pges-1 GFP::daf-16a)) hermaphrodite L4 larva 54 hours post synchronization at L1 larva starved after bleaching stage whole organism genetically modified (insertion) using bombardment targeting C. elegans daf-16
- Description
- Transgenic C. elegans line, stably expressing a C-terminal GFP-FLAG tag fused to DAF-16
- Life stage
- L4 larva
- Age
- Unknown
- Synchronization timepoint
- L1 larva starved after bleaching + 54 hours
Attribution
modERN project
- Lab
- Valerie Reinke, Yale
- Award PI
- Robert Waterston, UW
- Submitted by
- Alec Victorsen
- Source
- Valerie Reinke
- Project
- modERN
- External resources
- Aliases
- valerie-reinke:DAF-16_GFP_ZM8745_L4_Rep2
Genetic modifications
Accession | Category | Purpose | Method | Nucleic acid delivery method | Site |
---|---|---|---|---|---|
ENCGM684JAP | insertion | tagging | bombardment |
|
Strain information
- Status
- released
- Accession
- ENCDO075QZP
- Aliases
- wesley-hung:ZM8745
- Species
- Caenorhabditis elegans
- Genotype
- daf-16(mu86);daf-2(e1370) III; hpSi15 (Pges-1 GFP::daf-16a)
- Sex
- hermaphrodite
- Strain name
- ZM8745
- External resources
Documents
immunofluorescence
- Caption
- DIC and GFP image of transgenic worm taken right before collection. Worms imaged using a Zeiss Axioplan 2 imaging epifluorescence microscope. Scale bars are 10 micron.
- Submitted by
- Alec Victorsen
- Lab
- Valerie Reinke, Yale
- Grant
- U41HG007355