ENCBS549MFH / cell line

Summary

Status
released
Term name
A549
Summary
Homo sapiens A549 cell line genetically modified (insertion) using transduction, genetically modified using CRISPRi (pgRNA) 14 days after the sample was treated with 2 μg/mL Puromycin for 2 days, 0.2 μg/mL Puromycin for 2 days
Culture start date
2019-05-25
Culture harvest date
2019-07-10

Attribution

ENCODE4 project
Lab
Bing Ren, UCSD
Award PI
Yin Shen, UCSF
Submitted by
Bin Li
Source
ATCC
Project
ENCODE
External resources
Aliases
bing-ren:BC19B_A549_D14_B1_BC104

Treatment details

Treatment
2 μg/mL Puromycin (CHEBI:17939) for 2 days
Type
chemical
Purpose
selection
Treatment
0.2 μg/mL Puromycin (CHEBI:17939) for 2 days
Type
chemical
Purpose
selection

Genetic modifications

Accession
Category
Purpose
Method
Nucleic acid delivery method
Site
ENCGM554RTSinsertionexpressiontransduction
ENCGM353IMQCRISPRicharacterizationCRISPR

Donor information

Status
released
Accession
ENCDO000AAZ
Aliases
encode:donor of A549, bradley-bernstein:Donor of A549 cells, john-stamatoyannopoulos:ATCC_A549
Species
Homo sapiens
Life stage
adult
Age
58 years
Sex
male
Ethnicity
European
External resources
References