1. Biosamples
  2. cell line
  3. Homo sapiens
  4. K562

ENCBS506IZD / cell line

Summary

Status
released
Term name
K562
Summary
Homo sapiens K562 cell line stably expressing C-terminal eGFP-tagged ETS2
Description
K562 cell line stably expressing a C-terminal LAP-tag containing eGFP, fused to ETS2

Attribution

ENCODE3 project
Lab
Kevin White, UChicago
Award PI
Michael Snyder, Stanford
Submitted by
Trupti Kawli
Project
ENCODE
Aliases
kevin-white:ETS2_GFP_INPUT
Submitter comment
This biosample is a pool of the input DNA isolated from the constituent cells, not a pool of the cells themselves.

Pooled from biosamples

2 biosamples
Accession
Type
Term
Summary
ENCBS413AAAcell lineK562Homo sapiens K562 cell line stably expressing C-terminal eGFP-tagged ETS2
ENCBS381AAAcell lineK562Homo sapiens K562 cell line stably expressing C-terminal eGFP-tagged ETS2

Genetic modifications

Accession
Category
Purpose
Method
Nucleic acid delivery method
Site
ENCGM144WJAinsertiontaggingstable transfection

Donor information

Status
released
Accession
ENCDO000AAD
Aliases
encode:donor of K562, bradley-bernstein:Donor of K562 cells
Species
Homo sapiens
Life stage
adult
Age
53 years
Sex
female
Health status
chronic myelogenous leukemia (CML)
External resources
References

Documents

immunoblot
Caption: immunoblot of GFP tagged ETS2, expressed in K562 cells
Attachment from submitter
Attachment from submitters
2013_Mar_04.jpg
Submitted by
Alec Victorsen
Lab
Kevin White, UChicago
FACs analysis
Caption excerpt: eGFP-ETS2 tagged cells were stained with a nuclear dye, Vybrant DyeCycle Ruby…
Attachment from submitter
Attachment from submitters
ETS2_GFP_Exp.jpg
Caption
eGFP-ETS2 tagged cells were stained with a nuclear dye, Vybrant DyeCycle Ruby Stain from Life technologies, and sorted by FACS. Shown are the cells from the GFP+ fraction quantified for their GFP and nuclear signal colocalization.
Submitted by
Alec Victorsen
Lab
Kevin White, UChicago
FACs analysis
Caption excerpt: eGFP-ETS2 tagged cells were stained with a nuclear dye, Vybrant DyeCycle Ruby…
Attachment from submitter
Attachment from submitters
ETS2_Nuc_Loc_GFP++.jpg
Caption
eGFP-ETS2 tagged cells were stained with a nuclear dye, Vybrant DyeCycle Ruby Stain from Life technologies, and sorted by FACS. Shown are the cells from the GFP++ fraction quantified for their GFP and nuclear signal colocalization.
Submitted by
Alec Victorsen
Lab
Kevin White, UChicago
FACs analysis
Caption excerpt: eGFP-ETS2 tagged cells were stained with a nuclear dye, Vybrant DyeCycle Ruby…
Attachment from submitter
Attachment from submitters
ETS2_Nuc_Loc_GFP+.jpg
Caption
eGFP-ETS2 tagged cells were stained with a nuclear dye, Vybrant DyeCycle Ruby Stain from Life technologies, and sorted by FACS. Shown are the cells from the GFP+ fraction quantified for their GFP and nuclear signal colocalization.
Submitted by
Alec Victorsen
Lab
Kevin White, UChicago